Antibodies |
Mouse anti-GS |
Millipore Sigma |
Cat# MAB302; RRID AB_2110656 |
Rabbit anti-PDGFRα |
Cell Signaling |
Cat# 3174; RRID AB_2162345 |
Rabbit anti-ASPA |
GeneTex |
Cat# GTX113389; RRID AB_2036283 |
Chicken anti-MBP |
Aves |
Cat# MBP; RRID AB_2313550 |
Mouse anti-NeuN |
Millipore Sigma |
Cat# MAB377; RRID AB_2298767 |
Rabbit anti-GS |
Abcam |
Cat# Ab73593; RRID AB_2247588 |
Mouse anti-CNP |
Millipore Sigma |
Cat# MAB326; RRID AB_2082608 |
Mouse anti-MBP |
Biolegend |
Cat# 808401; RRID AB_2564741 |
Mouse anti-Gapdh |
Santa Cruz |
Cat# Sc-32233; RRID AB_627679 |
Rabbit anti-Actin |
Sigma |
Cat# A5060; RRID AB_2766001 |
Donkey anti-Rabbit |
Li-Cor |
Cat# 926–32213; RRID AB_621848 |
Donkey anti-Mouse |
Li-Cor |
Cat# 926–68072; RRID AB_10953628 |
Chemicals, Peptides, and Recombinant Proteins |
L-Methionine sulfoximine (‘MSO’) |
Sigma |
Cat# M5379 |
L-glutamine |
Sigma |
Cat# G3126 |
Critical Commercial Assays |
Glutamate Colorimetric Assay Kit |
Biovision |
Cat# K629 |
Glutamine Colorimetric Assay Kit |
Biovision |
Cat# K446 |
Ammonia Colorimetric Assay Kit |
Biovision |
Cat# K370 |
Glutamate Dehydrogenase Activity Assay Kit |
Abcam |
Cat# Ab102527
|
QuantiChrom Glutathione (GSH) Assay Kit |
Bioassays |
Cat# DIGT-250 |
Experimental Models: Organisms/Strains |
Mouse: Aldh1L1-eGFP
|
MMRRC |
OFC789Gsat/ 011015-UCD |
Mouse: MOBP-eGFP
|
MMRRC |
IN1Gsat |
Mouse: CX3CR1-eGFP
|
Jackson Laboratory |
JAX 005582 |
Mouse: MOG-iCre
|
Dr. Ari Waisman |
Hövelmeyer et al., 2005 |
Mouse: GSfl/fl
|
Dr. Wouter Lamers |
Heetal., 2010 |
Oligonucleotides |
See Table S1
|
n/a |
n/a |
Software and Algorithms |
FIJI |
NIH |
https://fiji.sc/; RRID SCR_002285 |
Microsoft Office 365 Excel |
Microsoft |
RRID SCR_016137 |
Prism 8 |
Graphpad |
RRID SCR 002798 |