KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GS | Millipore Sigma | Cat# MAB302; RRID AB_2110656 |
| Rabbit anti-PDGFRα | Cell Signaling | Cat# 3174; RRID AB_2162345 |
| Rabbit anti-ASPA | GeneTex | Cat# GTX113389; RRID AB_2036283 |
| Chicken anti-MBP | Aves | Cat# MBP; RRID AB_2313550 |
| Mouse anti-NeuN | Millipore Sigma | Cat# MAB377; RRID AB_2298767 |
| Rabbit anti-GS | Abcam | Cat# Ab73593; RRID AB_2247588 |
| Mouse anti-CNP | Millipore Sigma | Cat# MAB326; RRID AB_2082608 |
| Mouse anti-MBP | Biolegend | Cat# 808401; RRID AB_2564741 |
| Mouse anti-Gapdh | Santa Cruz | Cat# Sc-32233; RRID AB_627679 |
| Rabbit anti-Actin | Sigma | Cat# A5060; RRID AB_2766001 |
| Donkey anti-Rabbit | Li-Cor | Cat# 926–32213; RRID AB_621848 |
| Donkey anti-Mouse | Li-Cor | Cat# 926–68072; RRID AB_10953628 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| L-Methionine sulfoximine (‘MSO’) | Sigma | Cat# M5379 |
| L-glutamine | Sigma | Cat# G3126 |
| Critical Commercial Assays | ||
| Glutamate Colorimetric Assay Kit | Biovision | Cat# K629 |
| Glutamine Colorimetric Assay Kit | Biovision | Cat# K446 |
| Ammonia Colorimetric Assay Kit | Biovision | Cat# K370 |
| Glutamate Dehydrogenase Activity Assay Kit | Abcam | Cat# Ab102527 |
| QuantiChrom Glutathione (GSH) Assay Kit | Bioassays | Cat# DIGT-250 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Aldh1L1-eGFP | MMRRC | OFC789Gsat/ 011015-UCD |
| Mouse: MOBP-eGFP | MMRRC | IN1Gsat |
| Mouse: CX3CR1-eGFP | Jackson Laboratory | JAX 005582 |
| Mouse: MOG-iCre | Dr. Ari Waisman | Hövelmeyer et al., 2005 |
| Mouse: GSfl/fl | Dr. Wouter Lamers | Heetal., 2010 |
| Oligonucleotides | ||
| See Table S1 | n/a | n/a |
| Software and Algorithms | ||
| FIJI | NIH | https://fiji.sc/; RRID SCR_002285 |
| Microsoft Office 365 Excel | Microsoft | RRID SCR_016137 |
| Prism 8 | Graphpad | RRID SCR 002798 |