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. 2019 May 31;14(5):e0217339. doi: 10.1371/journal.pone.0217339

Fig 6. Ws-Lynx1 activity on A549 cells is mediated by interaction with α7-nAChRs.

Fig 6

(A). Representative histograms of cell distribution according to intensity of TRITC-labeled α-bungarotoxin for untreated cells (left) and cells with the blocked α7-nAChR expression by siRNA (right). (B). Median fluorescence intensities for TRITC-labeled α-bungarotoxin in untreated cells and cells with the blocked α7-nAChR expression by siRNA. Data are presented as mean ± SEM, n = 6. * (p<0.05) indicates the significant difference between the groups by two-tailed t-test. (C). Influence of ws-Lynx1, ws-Lynx1 in the presence of siRNA against α7-nAChR, the ws-Lynx1 R38A mutant, and recombinant neurotoxin II on the viability of A549 cells. The data are mean ± SEM, n = 7–11; #### (p<0.0001) indicates the significant difference from the control (100%) by a two-tailed one sample t-test. The control level (100% of viable cells) corresponds to the untreated cells and is shown by the dashed line.