Fig 6. Representation of the optimal ranges of urea concentration (a), NaCl concentration (b) and hybridization temperature (c) predicted, for each bacterium, through the response surface methodology, using a universal Eubacteria LNA/2’OMe probe (EUB388).

Ranges have been stablished assuming a fluorescence intensity of at least 85% of the maximum value. Dash lines represent the conditions selected to be included in initial optimization of LNA/2’OMe-FISH experiments (2 M of urea, 4 M of NaCl and 62 °C of hybridization temperature).