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. 2019 May 20;13(5):e0007418. doi: 10.1371/journal.pntd.0007418

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© 2019 Cámara et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A) Indirect immunofluorescence assays of permeabilized epimastigotes over-expressing TcSMUG S (TcSMUG S ox) from the indicated parasite clone revealed with mAb anti-FLAG. B) Lysates from TcSMUG S ox epimastigotes from the indicated parasite clone were fractionated on to anti-FLAG-Sepharose and input, flow-through (FT) and bound (B) fractions were probed with an anti-FLAG polyclonal antibody. C) Aliquots of the FT and B fractions were probed either with mAb 2B10 or mAb 10D8, recognizing Galp- and Galf-based glycotopes restricted to T. cruzi Gp35/50 kDa mucins, respectively. In B and C, the positions of relative molecular mass markers (in kDa) are shown.