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. 2019 May 20;13(5):e0007422. doi: 10.1371/journal.pntd.0007422

Fig 3. Silencing of Aae syt expression in the larval brain following syt.427 consumption.

Fig 3

The high levels of Aae syt expression detected throughout the A. aegypti L4 brain (control-treated larvae shown in A) were significantly reduced in larvae fed with dried inactivated yeast interfering RNA syt.427 tablets (B). Mean gray value results from three biological replicate experiments were compiled (C; n = 60 for syt.427-treated brains; n = 55 for control-treated brains), and these data were evaluated with a paired t-test (C). *** = P<0.001 when compared with control-fed larvae; data are represented as average mean gray values, and error bars denote SEM. Representative brains from these experiments are oriented dorsal upward in this figure. LAL: larval antennal lobe; OF: olfactory foramen; OL: optic lobe; SOG: sub-esophageal ganglion; SuEG: supra-esophageal ganglion. Scale Bar = 100 μm.