Skip to main content
. 2019 May 20;13(5):e0007422. doi: 10.1371/journal.pntd.0007422

Fig 4. Neural defects observed in the L4 brains of larvae fed with yeast interfering RNA larvicide syt.427.

Fig 4

Stage L4 larval brains were labeled with mAbnc82 (white in A1, B1; red in A2, B2), which labels synaptic active zones. nc82 levels were significantly reduced (C) in the synaptic neuropil of L4 larvae that had consumed syt.427 (B1, B2; compare to white staining of control brain in A1/red staining in A2), but levels of TO-PRO, which marks nuclei, were not significantly different (C) between syt.427- (B2) and control-treated (A2) larvae. Data compiled from three biological replicate experiments containing a total of 80 syt.427-treated and 49 control-treated larvae are shown and were evaluated with a paired t-test (C). Data are represented as average mean grey values, and error bars denote SEM (C). *** = P<0.001 when compared with control-fed larvae. Representative brains from these experiments are oriented dorsal upward in this figure. LAL: larval antennal lobe; OF: olfactory foramen; OL: optic lobe; SOG: sub-esophageal ganglion; SuEG: supra-esophageal ganglion. Scale Bar = 100 μm.