Table 1. Antibodies used for flow cytometry.
Target Marker (cells) | Fluorochrome | Clone | Reference | Supplier |
---|---|---|---|---|
CD11b (macrophages) | VioBlue | M1/70 | 48-0112-82 | eBioscience |
Ly6G (neutrophils) | FITC | 1A8 | 127606 | BioLegend |
CD11c (dendritic cells) | PE | N418 | 12-0114-83 | eBioscience |
Ly6C (monocytes) | PerCP/Cy5.5 | HK1.4 | 45-59-32 | BioLegend |
F4/80 (macrophages) | APC | BM8 | 123116 | BioLegend |
F4/80 (macrophages) | PE-CY5 | BM8 | 15–4801 | eBioscience |
NK1.1 (NK cells) | VioBlue | PK136 | 108732 | BioLegend |
CD8 (T cells) | FITC | 53–6.7 | 53-0081-82 | eBioscience |
CD4 (T cells) | PerCP | RM4-5 | 553052 | eBioscience |
CD3 (T cells) | PE-Cyanine7 | 145-2C11 | 25-0031-82 | eBioscience |
CD19 (B cells) | APC | MBI9-1 | 17-0191-82 | eBioscience |
CD16/CD32 (Fc Block) | 14-0161-86 | eBioscience | ||
iNOS | PE | CXNFT | 12-5920-80 | eBioscience |
IFN-γ | APC | XMG1.2 | 17-7311-82 | eBioscience |