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. 2019 May 31;14(5):e0217552. doi: 10.1371/journal.pone.0217552

Fig 1. Negative controls of kidney transversal sections from SHR animals.

Fig 1

Representative photomicrographs of kidney sections from 4 SHR rats incubated in parallel with 10% normal horse serum instead of the primary antibody to assess the level of background ascribed to nonspecific binding of the secondary antibody. A clean background was obtained in superficial (SG) and deep (DG) glomeruli, proximal (PCT) and distal (DCT) convoluted tubule, loop of Henle (LH) or collecting tubule (CT). Scale bar: 20 μm.