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. 2019 May 31;14(5):e0217755. doi: 10.1371/journal.pone.0217755

Fig 3. Tpi gene segments showing polymorphic sites diagnostic for strain identity or that define important haplotypes.

Fig 3

The locations of primers used for PCR amplification and DNA sequencing are provided (←, →) as are the locations of the TpiE4 and TpiI4 segments (—). (A) The gTpi183Y site is diagnostic for TpiC and TpiR strain designations as well as TpiH heterozygotes, while polymorphisms at gTpi192Y and gTpi198Y identify within strain variants. The TpiI4 segment is defined by sites 10 and 172 within the intron. (B) Sequences of the four TpiI4 haplotypes found in India and South Africa collections. Letters in dark bold identify observed sites of polymorphism in these collections. Site 131 was associated with consistently poor sequence quality and so was assumed to be the consensus G for all specimens.