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. Author manuscript; available in PMC: 2019 Aug 20.
Published in final edited form as: Nature. 2019 Feb 20;567(7747):194–199. doi: 10.1038/s41586-019-0953-5

Extended Data Figure 2 |. DncV, cGAS, and CdnE reaction order.

Extended Data Figure 2 |

a–c, Incubation of CD-NTase enzymes with nonhydrolyzable nucleotides traps reaction intermediates and identifies reaction order. Left, PEI-cellulose TLC analysis of reactions as in Fig. 1b where individual NTPs have been replaced with nonhydrolyzable nucleotide analogs. Exposed gamma phosphates are labeled in parenthesis to indicate these are removed by phosphatase treatment prior to analysis. Data are representative of 3 independent experiments. Right, previously determined reaction mechanisms (DncV and cGAS)10,51 and proposed reaction mechanism for CdnE.