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. Author manuscript; available in PMC: 2020 Jun 30.
Published in final edited form as: J Proteomics. 2019 May 4;202:103374. doi: 10.1016/j.jprot.2019.05.002

Figure 1.

Figure 1

VacA protein and transcript levels are increased in response to high salt concentration. H. pylori was cultured in Brucella broth filtrate supplemented with cholesterol and containing the indicated concentrations of NaCl (0.25%, 0.5%, 1.0% and 1.25%). (A) Western blot analysis of VacA in culture supernatant samples from 24 and 36 h time points (standardized at 40 μg protein per sample). (B) Cultures were inoculated at an OD600 of about 0.3, and samples from a 1 h time point (100 μg protein per sample) were analyzed by Western blotting. The results are representative of analyses of three independent sets of cultures. (C) H. pylori was cultured for 1 h in Brucella broth-cholesterol containing 0.5% NaCl or 1.25% NaCl. Transcript abundance was analyzed by RT-qPCR. Normalized transcripts signals from bacteria grown in 1.25% NaCl were compared to those from bacteria grown in 0.5% NaCl. RNA was isolated from four independent experiments. The mean and standard error of the mean are shown. A significant difference (P < 0.05, 1-way ANOVA) was observed when comparing the effect of high salt on vacA transcript levels, compared to effects on control genes (gyrB and atpA).