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. Author manuscript; available in PMC: 2020 Jun 30.
Published in final edited form as: J Proteomics. 2019 May 4;202:103374. doi: 10.1016/j.jprot.2019.05.002

Table 1.

Proteins selectively released into the extracellular space under ≥5 culture conditions

Gene/Protein Description Enrichment of proteins in culture supernatant compared to the soluble bacterial fraction under the indicated culture conditions a,b
0.5 % NaCl 1.0% NaCl 1.25% NaCl Average Esupc Predicted cleavage sited
HP0104*e 2',3'-cyclic-nucleotide 2'-phosphodiesterase (CpdB) 5.88 6.08 6.28 6.08 18–19
HP0129* hypothetical protein 2.64 2.71 5.54 3.63 21–22
HP0175 cell binding factor 2; peptidyl prolyl cis,transisomerase 1.22 1.85 2.18 1.75 26–27
HP0176 fructose-bisphosphate aldolase (Tsr) 1.34 1.53 1.23 1.37 -
HP0211* hypothetical secreted protein (HcpA) 2.74 4.99 3.64 3.79 25–26
HP0224 peptide methionine sulfoxide reductase (MsrA) 0.94 1.30 1.45 1.23 22–23
HP0231* hypothetical protein; DsbK (thioloxidoreductase) 2.04 2.51 2.81 2.45 26–27
HP0235* hypothetical secreted protein (HcpE) 3.57 4.61 3.83 4.00 24–25
HP0298* dipeptide ABC transporter, periplasmic dipeptide-binding protein (DppA) 2.74 4.38 3.87 3.66 22–23
HP0304* alginate lyase 5.10 3.86 5.12 4.69 19–20
HP0323* membrane bound endonuclease (Nuc); NucT 2.96 2.95 3.65 3.19 16–17
HP0377* thiol:disulfide interchange protein (DsbC), putative 2.92 4.65 5.03 4.20 24–25
HP0389 superoxide dismutase (SodB) 1.60 1.23 1.54 1.46 -
HP0410* putative neuraminyllactose-binding hemagglutinin homolog (HpaA paralog) 4.49 3.97 4.36 4.27 24–25
HP0485* catalase-like protein 2.97 3.31 4.25 3.51 24–25
HP0630 modulator of drug activity (Mda66) 1.51 1.72 1.67 1.63 -
HP0657* processing protease (YmxG) 2.34 2.68 2.63 2.55 20–21
HP0871* CDP-diglyceride hydrolase (Cdh) 3.81 4.14 5.05 4.33 21–22
HP0953* hypothetical protein 1.98 2.93 3.22 2.71 20–21
HP0973* hypothetical protein 2.35 4.25 3.71 3.44 28–29
HP1012* protease (PqqE) 2.46 2.15 2.54 2.38 26–27
HP1019* serine protease (HtrA) 4.89 5.60 5.84 5.44 -
HP1098* hypothetical secreted protein (HcpC) 3.08 3.63 3.93 3.55 25–26
HP1118* gamma-glutamyltranspeptidase (Ggt) 2.81 3.52 3.83 3.39 27–28
HP1164 thioredoxin reductase (TrxB); flavodoxin: quinone reductase (FqrB) 1.80 1.49 1.79 1.69 -
HP1173* hypothetical protein 3.77 5.79 6.96 5.51 26–27
HP1186* carbonic anhydrase 1.97 4.16 3.87 3.33 18–19
HP1227* cytochrome c553 1.81 2.99 3.13 2.64 19–20
HP1285* 5’-nucleotidase, lipoprotein e(P4) family 2.90 3.48 4.15 3.51 -
HP1286* polyisoprenoid-binding protein (YceI) 1.92 2.18 2.90 2.34 17–18
HP1454* Lpp20 domain containing protein 2.39 3.54 5.03 3.65 19–20
HP1458 thioredoxin-2 (Trx 2) 1.69 1.40 1.79 1.63 -
HP1561* iron(III) ABC transporter, periplasmic iron-binding protein (CeuE1) 2.52 2.89 3.09 2.83 31–32
HP1562* iron(III) ABC transporter, periplasmic iron-binding protein (CeuE2) 2.62 2.98 3.25 2.95 29–30
HP1564* putative outer membrane protein 2.21 2.45 2.79 2.48 -
Averagef 2.59 3.17 3.52 3.09
a

Esup values (log2) were calculated and analyzed as described in Methods. The reported data represent the average of Esup values (log2) from broth cultures grown under the indicated conditions for 24 and 36 h. All proteins listed in the table were selectively released in at least 5 of the 6 conditions tested (see Supplemental Table 1).

b

Raw data with complete list of selectively released proteins (1 or more condition) are shown in Supplementary Table 1.

c

Average Esup values (log2) for each protein, based on analysis of 6 culture conditions.

d

Predicted signal sequence cleavage site, based on use of SignalP 5.0 [71]. Numbers represent positions of amino acids at the site of predicted cleavage. Dash (−) indicates that no signal sequence is predicted.

e

Asterisks (*) represent proteins also selectively released in Snider et al. [37]

f

Average Esup value for all of the listed proteins.