(A) PS exposure on EVs determined with a prothrombinase assay was significantly higher in AF (n=10) compared with plasma from healthy controls (n=7). (B) EV-TF activity determined with a factor Xa generation assay was significantly higher in AF (n=10) compared with plasma from cancer patients with overt DIC (n=10). EV-TF activity was not detectable in plasma from healthy controls (n=7). Measurements were performed in duplicates. EV, extracellular vesicles; TF, tissue factor; PS, phosphatidylserine; AF, amniotic fluid; DIC, disseminated intravascular coagulation.