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. Author manuscript; available in PMC: 2019 Oct 24.
Published in final edited form as: Nature. 2019 Apr 24;569(7754):66–72. doi: 10.1038/s41586-019-1146-y

Figure 4. Transient Yap1 activation is necessary for symmetry breaking.

Figure 4

a, Yap1 localization (Lgr5::DTR-EGFP). b, Yap1 protein abundance along pseudotime (n=6892, n=organoids). c, Efficiency of organoids formation from cells isolated at 72h and at 120h (normalization: 120h). Fixed: 72h (n=4, n=replicates, two-sided t-test, p-value 0.00007). d, Efficiency of organoid formation in Verteporfin (normalization: control). Fixed: 48h (n=2, n=replicates). e, Efficiency of organoid formation from Yap1 KO (normalization: control). Fixed: 72h (n=8, n=replicates, two-sided t-test, p-value 0.002). f, Efficiency of organoid formation from Yap1 overexpression and Ereg activation (normalization: control). (n=4, n=replicates, two-sided t-test, p-value: Yap1OE-0.001, Ereg-0.0002). g, Images of organoids treated with Verteporfin (at 48h), Yap1-overexpression and Ereg. Top panel: fixed at 72h. Bottom panel: fixed at 96h (Yap1-overexpression) or 120h (Control, Verteporfin, Ereg). h, Quantification of enterocysts as retrieved in g. (normalization: control). (n=4, n=replicates, two-sided t-test, p-value Verteporfin-0.01, Yap1 OE-0.002, Ereg-0.05). (c, d, e, f, h) Barplots: mean ± s.d.