Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 31;9:8120. doi: 10.1038/s41598-019-44542-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PbpTKL is dispensable for intra-erythrocytic cycle completion, gametocyte emergence and male gametocyte exflagellation. (a–e) Data collected using mice infected either with the wild-type PbGFP strain (parental) or the PbGEM-342364-transfected strain (pGEM) – 10⁶ parasites/mouse, seven mice per group. (a) Knocking out PbpTKL does not impact parasitemia. Parasitemia follow-up in mice infected either with the parental or pGEM P. berghei parasites (mean ± SD). (b) Knocking out PbpTKL does not improve mouse survival. Percent survival of mice infected with either parental or pGEM P. berghei parasites. (c) Knocking out PbpTKL does not affect gametocyte emergence. Gametocytemia follow-up in mice infected with either with the parental or pGEM P. berghei parasites (mean ± SEM). (d) Knocking out PbpTKL does not affect male/female gametocyte ratio. Male/female ratios were evaluated in mice infected either with the parental or pGEM P. berghei parasites (data points and means ± SD are shown). (e) Knocking out PbpTKL does not affect male gametocyte exflagellation. Exflagellation center numbers were assayed for parental and pGEM parasites at a parasitemia of 10–20% and > 20% (data points and means ± SD are shown). (f–h) Data collected using cloned pG230 P. berghei parasites expressing PbpTKL-AID-HA, after incubation with or without the auxin 3-idoleacetic acid (IAA). (f) PbpTKL-AID-HA is degraded in vitro in the presence of IAA. PbpTKL-AID-HA parasites were incubated either with (+IAA) or without IAA (−IAA). After total lysis of incubated parasites, immunoprecipitation assays (IP) were performed using anti-HA agarose beads. PbpTKL-AID-HA was detected by western blot (WB) using anti-HA antibodies. Uncropped blots are available in the Supplementary Information. (g) Inducible knockdown of PbpTKL does not prevent completion of the intra-erythrocytic cycle. In vitro follow-up of PbpTKL-AID-HA parasite stages after synchronized infection in mice (five per group) – parasites were cultured in the presence (+IAA) or absence (−IAA) of IAA, hpi = hours post-intravenous injection of Nycodenz-enriched schizonts (mean ± SD). (h) Inducible knockdown of PbpTKL does not affect male gametocyte exflagellation. Exflagellation centers were assayed for PbpTKL-AID-HA parasites after incubation with (+IAA) or without IAA (−IAA) (mean ± SD). ns, no significant difference.