Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 31;9:8140. doi: 10.1038/s41598-019-44597-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Single molecule analysis of nucleosome binding at bivalent promoters reveal alternative configurations within mESCs cell population. (A) Scatter plot with trendline showing population-averaged nucleosome occupancy (red) and DNA methylation (black) relative to the TSS of a bivalent gene (Wnt1) analyzed by NOMe-PCR. Nucleosome occupancy is calculated as the percentage of unmethylated GpC sites. DNA methylation is plotted as the percentage of methylated CpG dinucleotides. Arrow indicates the direction of transcription. (B) Lollipop diagram showing DNA methylation and nucleosome occupancy at the single molecule for Wnt1 promoter region. Top panels show cytosine methylation at CpGs (white for unmethylated, black for methylated) and bottom panels show methylation patterns at GpC dinucleotides (white for unmethylated, blue for methylated). Red bars highlight GpC unmethylated regions long enough to accommodate a nucleosome. NDRs are marked with a grey box. (C) Scatter plot with trendline showing population-averaged nucleosome occupancy (red) and DNA methylation (black) relative to the TSS of the bivalent gene Gata4 analyzed by NOMe-seq. Arrow indicates the direction of transcription. (D) Lollipop diagram showing DNA methylation and nucleosome occupancy at the single molecule for Gata4 promoter region. Genomic coordinates are indicated in the analysis of Gata4 by NOMe-seq in (D) but not in analysis of Wnt1 by NOMe-PCR in (B).