FIG 2.
Transcriptome analysis of genes in strain DM1 in response to DMF. (A) Total numbers of genes showing upregulation or downregulation within the chromosome and the plasmid pLVM1. (B) Heat map of the hierarchical cluster analysis of gene expression in strain DM1. RNA_DMF, treatment group cultured with 2,000 mg liter−1 DMF; RNA_Gly, control group cultured with 1% (vol/vol) glycerol. (C) Heat map of putative genes predicted to be involved in DMF degradation according to RNA-seq data. The formula used to obtain the data for drawing the heat map was as follows: log2 basemean RNA_Gly − [(log2 basemean RNA_Gly + log2 basemean RNA_DMF)/2]. (D) KEGG pathway enrichment analysis of differentially expressed genes in response to DMF.