TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source |
|---|---|---|
| Methylobacterium sp. | ||
| DM1 | DMF-degrading strain; Gram negative | CCTCC (accession no. M2018663) |
| DM1ΔpLVM1 | Strain DM1 depleted of plasmid pLVM1 | This study |
| Escherichia coli | ||
| DH5α | F recA1 endA1 thi-1 hsdR17 supE44 relA1 deoR(lacZYA-argF) U169 80dlacZM15 | TransGen |
| BL21(DE3) | F− ompT hsdS(rB− mB−) gal dcm(DE3) | TransGen |
| Plasmids | ||
| pETDuet-1 | Ampr; expression vector | |
| pETDuet-1-dmfA1 | Ampr; pETDuet-1 containing dmfA1 | This study |
| pETDuet-1-dmfA2 | Ampr; pETDuet-1 containing dmfA2 | This study |
| pETDuet-1-dmfA1A2 | Ampr; pETDuet-1 containing dmfA1 and dmfA2 | This study |
a
Ampr, ampicillin resistance.