Figure 6.
MTMR3 and MTMR4 are required for PtdIns3P production and suppression of STING pathway. A, confocal images of PX p40phox (PtdIns3P) and PH DOK5 (PtdIns5P) localization in control and DKO cells. Scale bar = 10 μm. B, number of YFP positive puncta in control and DKO cells was counted. C and D, HEK293T cells were overexpressed with MTMR3 and MTMR4 expression plasmids along with an expression plasmid for STING, IPS-1, MyD88, or TRIF and a reporter plasmid driven by the IFNβ promoter. After 24 h, cells were lysed and the luciferase expression was measured. E, control and DKO cells were stimulated with ISD, poly(I:C), or LPS for the indicated time points. Cells lysates were subjected to WB with the indicated antibodies. Data are represented as mean ± SE. *, p < 0.05 (Student's t-test).