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. 2019 Apr 8;294(21):8640–8652. doi: 10.1074/jbc.RA118.007006

Figure 4.

Figure 4.

HDAC5 knockdown stimulates NRF2 target gene expression in cardiomyocytes. A–D, NRVMs were infected with adenoviruses (m.o.i. = 10) encoding nontargeting control shRNA (shCtrl) or shRNAs targeting HDAC4 (shHD4), HDAC5 (shHD5), and HDAC9 (shHD9). After 72 h of infection, total RNA was isolated, and Hdac4 (A), Hdac5 (B), Hdac9 (C), and Hmox1 (D) mRNA levels were determined by qRT-PCR. E, protein homogenates were prepared from NRVMs infected with adenoviruses encoding shCtrl or shHD5 for 72 h. Immunoblotting was performed with antibodies against the indicated proteins. F, NRVMs were infected with adenoviruses encoding shCtrl or shRNAs targeting two distinct regions of the Hdac5 mRNA transcript (shHD5(#1) and shHD5(#2)) for 72 h prior to preparing total RNA for qRT-PCR analysis of Srxn1 mRNA expression. For all graphs, values represent means + S.E.; *, p < 0.05 versus shCtrl.