Figure 1.

Cited2 deficiency protects against death and Cited2 ectopic expression induces death after hypoxia/reoxygenation in cortical neurons. A, cortical neurons from Cited2 WT and KO littermates (specified n) were exposed to hypoxia/reoxygenation. Neuronal survival was estimated by assessment of nuclei morphology under the microscope. B, cortical neurons were infected by AAV-Cited2: GFP or GFP only–expressing particles and 10 days after that subjected to hypoxia/reoxygenation. Survival was estimated by assessment of nuclei morphology of GFP-positive cells after fixation. Three independent experiments were performed. All stats were done by two-way ANOVA, followed by Tukey's post hoc test. NT, nontreated; H24, 24-h hypoxia; R12 and R24, 12 and 24 h reoxygenation.