Figure 2.

After genotoxic and hypoxic insults, Cited2 and E2F1 levels are up-regulated, whereas E2F4 levels decrease. C57Bl/6 primary cortical neurons were submitted to DNA damage (A) or hypoxia/reoxygenation (B). Protein expression profiles for Cited2, E2F1, and E2F4 were assessed for both models. Representative Western blotting images are shown to the left, and quantification of signals was performed by ImageJ densitometry. The graphs to the right represent average protein changes using specified n independent experiments. Campto, 10 μm camptothecin treatment. The data were analyzed using ANOVA followed by Tukey's test. NT, nontreated; Reox., reoxygenation.