Figure 6.
Identification of residues in the N-terminal region of cPγ that enhance Gtα*-GTPγS activation efficiency. A and B, four N-terminal truncated cPγ mutants (cPγ5–83, cPγ9–83, cPγ13–83, and cPγ15–83) were reconstituted with Pαβ to test the Gtα*-GTPγS activation efficiency of PDE6. C, three internal deletion mutants of cPγ (cPγ1–4_9–83, cPγ1–4_15–83, and cPγ 1–8_15–83) were also tested under the same experimental conditions. The Gtα*-GTPγS activation assay was carried out and analyzed as described in the legend for Fig. 3A, and the results are summarized in Table 1.