Figure 4.

Knockdown of NOTCH1 induces an increase in cell death and a decrease in cell proliferation. A, C4-2R cells were infected with shCtrl or shNOCH1 virus particles and selected with puromycin. Cells were collected and subjected to Western blotting analysis with indicated antibodies. B, stable C4-2R shCtrl and shNOTCH1 cells were treated with DMSO or 40 μm enzalutamide for 72 h and subjected to Western blotting analysis. C, stable C4-2R shCtrl and shNOTCH1 cells were treated with DMSO or increasing concentrations of enzalutamide and subjected to MTT assay. Optical densities were measured and normalized to DMSO-treated samples. *, p < 0.05; **, p < 0.01.