Figure 5.

Inhibition of Notch signaling pathway resensitizes enzalutamide-resistant cells to enzalutamide and causes cell death and decreased colony formation ability. A, C, and E, MR49F, C4-2R, and 22RV1 cells were treated with DMSO, PF-03084014, enzalutamide, or both at the indicated concentrations for 72 h. The cells were collected and subjected to Western blotting analysis. B, D, and F, low seeding density MR49F, C4-2R, and 22RV1 cells were treated with DMSO, PF-03084014, enzalutamide, or both at the indicated concentrations until colonies were formed. The cells were fixed with 4% formaldehyde and stained with crystal violet.