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. 2019 Apr 10;294(21):8384–8394. doi: 10.1074/jbc.RA118.006508

Figure 2.

Figure 2.

C5aR2 deficiency restricts NLRP3 inflammasome activation in vivo. A and B, C5aR2+/+ and C5aR2−/− mice were injected i.p. with 1 mg of MSU, and the level of IL-1β (A) and peritoneal exudate cell numbers (B) in the peritoneal lavage fluids were measured. C, C5aR2+/+ and C5aR2−/− mice were injected i.p. with LPS (10 mg/kg body weight) for 12 h, and the serum levels of IL-1β (left panel), TNFα (center panel), and HMGB1 (right panel) were measured. D, the survival of C5aR2+/+ and C5aR2−/− mice subjected to 10 mg/kg LPS was monitored. Data points represent individual animals. Data were combined from three independent experiments with n = 15 mice/group (A–C) and shown as mean ± S.D. Two-tailed Student's t test was used (A–C). *, p < 0.05; ***, p < 0.001; ns, not significant. The survival data in D are representative of two independent experiments with n = 20 mice/group and presented as Kaplan–Meier plot by log-rank (Mantel–Cox) test.