Figure 4.

PKR rescues NLRP3 inflammasome activation in the absence of C5aR2. A–C, peritoneal macrophages from C5aR2^+/+ and C5aR2^−/− mice were electroporated with a PKR expression vector or an empty vector. 24 h later, cells were subjected to NLRP3 canonical stimuli as indicated. The transfection efficiency was quantified by Q-PCR (A), and the expression of IL-1β and TNFα (B) and HMGB1 (C) was measured. The results represent the mean ± S.D. of three independent experiments performed in triplicate. Two-tailed Student's t test was used. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. Ctrl, control.