Figure 5.

PKR is involved in the production of C5a-induced proinflammatory mediators in the blood. A and B, ELISA of serum IL-1β, TNFα (A), and HMGB1 (B) in PKR^+/+ and PKR^−/− mice after i.p. injection of 10 mg/kg LPS with or without C5a (2.5 μg per mouse) for 12 h. Data points represent individual animals. Data were combined from three independent experiments with n = 15 mice/group and are shown as mean ± S.D. Two-tailed Student's t test was used. **, p < 0.01; ***, p < 0.001; ns, not significant.