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. 2019 Apr 2;294(21):8480–8489. doi: 10.1074/jbc.RA118.006040

Figure 4.

Figure 4.

WT-M013 and mutants modulate the caspase-1 activation and cytokine response in THP-1 cells. A and B, luminescence assay result to quantitate the caspase-1 activation in THP-1 cell supernatants transfected with V5-tagged WT-M013 and mutant plasmids and infected with vMyx-M013KO for 6 h (A) and 24 h (B) (as described under “Results”). C–E represent the ELISA results for TNF, IL-6, and IL-18 in the supernatants from THP-1 cells transfected with V5-tagged WT-M013 and mutants and infected with vMyx-M013KO for 6 h. F–H, ELISA results for TNF, IL-6, and IL-18 in the supernatants from THP-1 cells transfected with V5-tagged WT-M013 and mutants and infected with vMyx-M013KO 24 h. WT, WT M013; C1, cells infected with vMyx-M013KO; M013PYD, M013 with C terminus deletion; E28Q, D29N, D57N, and D41A, M013 with mutations E28Q, D29N, D57N, and D41A, respectively; C2, cells with no transfection or infection (negative control); LPS, positive control; P, cells transfected with an empty plasmid. Significance was tested using one-way analysis of variance, with multiple comparison correction. A value of p < 0.05 was considered significant.