Figure 4.

Immunoblot analysis of the conformational and sequence specificity of monovalent and bivalent AF1 antibodies. Dilutions of Aβ, tau, and α-synuclein (αSyn) (disaggregated (D) and fibril (F)) were deposited on nitrocellulose membranes. Binding of monovalent (scFv) and bivalent AF1 (scFv–Fc) antibodies were evaluated at 100 nm (scFv) and 10 nm (scFv–Fc) in 5% milk. A sequence-specific anti-Aβ antibody (NAB228, 1:1000 dilution, 5% milk) was also evaluated for comparison. Immunoblots were imaged using X-ray film (1–20 s of exposure time). The loading control blot was detected using silver colloidal stain.