Figure 8.

Bivalent AF1 antibodies display high conformational specificity for Aβ fibrils. A and B, conformational specificities of AF1 antibodies for Aβ fibrils relative to disaggregated Aβ were evaluated for two bivalent formats of AF1 (Fc fusion (A) and 150-amino acid linker (B)) relative to monovalent AF1. The flow cytometry analysis was performed using soluble AF1 antibodies and immobilized Aβ, and antibody binding is reported in terms of relative fluorescent units (RFU). The monovalent and bivalent AF1 antibodies in B have an additional N-terminal (StrepTag-II) tag relative to the AF1 antibodies in A. C, apparent conformational specificity ratios for the AF1 antibodies. The ratios are reported as the signals for AF1 binding to Aβ fibrils relative to those for AF1 binding to disaggregated Aβ. In A and B, three independent experiments were performed, and results from a representative experiment are shown. In C, average values for 2–4 independent experiments are shown, and the error bars are standard errors.