Figure 1.
Reduced EZH2 expression during adipogenic differentiation. A and B, time course of EZH2 protein expression during in vitro adipogenic differentiation of human SQ preadipocytes (n = 4). Protein expression was determined by Western blotting (A) and quantified by densitometry analysis (B). Representative Western blotting image (C) and densitometry analysis (D) of EZH2 and RbAP48 proteins in isolated MA compared with SV fraction from mouse SQ adipose tissues. Individual protein levels were normalized to total protein, respectively (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. D0, undifferentiated; D3, 3 days post-adipogenic differentiation; D6, 6 days post-adipogenic differentiation. SV, stromal vascular; MA, mature dipocytes. Error bars, S.E.