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. 2019 Apr 11;294(21):8606–8616. doi: 10.1074/jbc.RA119.007554

Figure 5.

Figure 5.

Measurements of the N dependence of invading strand extension in a D-loop resulting from RecA-mediated heteroduplex product formation when DNA synthesis is performed by DNA LF-Bsu Pol. A, ΔΔF(t) versus time curves for ΔL = −1 and for N = 82 (blue), N = 50 (green), N = 20 (magenta), and N = 0 (black). The experimental schematic is shown in Fig. 4A. The yield of outgoing strand formation is 14% for N = 82 and 50 and 6% for N = 20. B, schematic of the experimental design using the same symbols as in previous figures. The dsDNA is 180 bp long, and fluorophores are positioned 11 (rhodamine) and 9 bp (fluorescein) from the end of the dsDNA. The 3′ end of the invading strands is 59 bp from the end of the dsDNA, yielding ΔL = −48. C, ΔΔF(t) versus time curves for ΔL = −48 and N = 82 (blue), N = 50 (green), N = 20 (magenta), and N = 0 (black). The yield of outgoing strand formation is ∼5% for N = 82 and 50 and ∼2% for N = 20.