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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Subcell Biochem. 2010;54:149–157. doi: 10.1007/978-1-4419-6676-6_12

Figure 2.

Figure 2

Pup conjugation. Following a deamidation reaction catalyzed by Dop, Pup is conjugated to a substrate (e.g., FabD) via an isopeptide linkage between a Lys of the substrate and either the C-terminal side chain carboxylate of Glu (i) or the C-terminal backbone carboxylate (ii). The ligation reaction is catalyzed by PafA and is ATP-dependent. Ligation is shown through the γ-carboxylate of Glu for simplicity.