Fig. 2.

rTBI induces RGC loss and CCL20 expression in the retina 7 days post-injury. a Representative low magnification, bright field image of H&E staining of cross section of the whole eye. Images, as shown in b and g, are representations of one of the boxed areas in the a. Cells were counted or integrated density was measured  from both the boxed areas, and the average numbers were plotted as the histogram shown in c and h respectively. b Photomicrograph of H&E staining of retinal sections from one of the boxed areas in a under different experimental conditions. Arrows indicate RGC loss. c Histogram showing the RGC counts from the H&E-stained sections. d Retinal flat mount preparation showing immunostaining with anti-RBPMS antibody. e Representative high-magnification immunofluorescence images of the boxed area in d showing RBPMS-positive ganglionic cells under different experimental conditions. f Histogram showing RBPMS positive cell counts under different experimental conditions. g Photomicrograph showing immunoperoxidase staining with anti-CCL20 antibody of the retinal sections from one of the boxed areas shown in a. Scale bar 100 μm, inset, high-magnification image of the boxed area of the ganglionic cell layer, scale bar, 20 μm. h ImageJ quantitation of CCL20 immunoreactivity in the retina. i CCL20 protein expression in the retina as measured by Quantikine ELISA. *p < 0.01, **p < 0.001, ***p < 0.0001