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Effect of anti-CCL20 antibody or CCR6 knock down on microgliosis and astrogliosis in optic nerve following rTBI. a Representative immunofluorescence images showing Iba1 (red) or GFAP (green) immunoreactivity in the optic nerve. Scale bar 100 μm. b, c Histograms showing average count of Iba1-positive cells (b) or average fluorescence intensity of GFAP immunoreactivity (c) as quantitated by ImageJ. Values are shown as mean ± SEM. *p < 0.01, **p < 0.001, ***p < 0.0001
