Fig. 2.

Schematic diagram of the methods used in this work for redirection of carbon flux. a The inducible gene silencing was triggered by expressing a hairpin RNA. After design and induction of the hairpin dsRNA, the dsRNA can be digested by the dicer enzyme in the cell to produce small interference RNA (siRNA) that triggers RNA interference (RNAi) to specifically degrade the endogenous target mRNA. b The short and long fragments of psy are constructed for conformation of the hairpin dsRNA under the control of tac promoter, as well as the exogenous genes coding TPS, PgpB and ATPS