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. 2019 May 31;20:440. doi: 10.1186/s12864-019-5837-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Relative tissue-specific expression of P450 transcripts, as analyzed via PCR and visualized in 3% agarose gels. Antenna Male (AM), Antenna Female (AF), Proboscis (S), Legs (L), Thorax (Tx), Abdomen (Ab) and Wings (W) indicate the source tissues. The expression of all P450s in the gut and fat body (FB) tissues was quantified via RT-qPCR, and the mean fold-changes in gene expression compared to tubulin and β-actin are provided under RQ. The oligonucleotide primer details, annealing temperature and amplification size (bp) are given in Additional file 1: Table S1. The 3 kb DNA ladder was used as a marker