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. 2019 May 31;10:157. doi: 10.1186/s13287-019-1270-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Snhg3 is enriched in mouse embryonic stem cells (mESCs). a qRT-PCR analysis of Snhg3 expression in mESCs and mouse embryonic fibroblasts (MEFs). To induce differentiation, mESCs were cultured without LIF (b), with 5 μM retinoic acid (RA) (c), or as embryoid bodies (EBs) (d) for the indicated amounts of time. Cells or EBs were collected for qRT-PCR to detect Snhg3 expression. Nanog was used as a positive control. Data are presented as mean ± SD; n = 3, two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001 for all panels