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. 2019 May 31;10:157. doi: 10.1186/s13287-019-1270-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Snhg3 is essential for mouse embryonic development. a Morphology of E3.5 mouse embryos after siControl or siSnhg3 treatment; × 10 objective. b siControl- or siSnhg3-injected zygotes were cultured in vitro until E4.5 and the blastocyst/non-blastocyst rate was quantified. siControl, n = 50; siSnhg3, n = 64. ***p < 0.001, chi-square test. c qRT-PCR analysis of Snhg3, Nanog, and Oct4 expression in blastocysts after siControl or siSnhg3 treatment. Data are presented as the mean ± SD; n = 3, two-way ANOVA. **p < 0.01, ***p < 0.001. d Immunostaining of Nanog (green) and Oct4 (red) in blastocysts after siControl or siSnhg3 treatment. The nuclei were stained with DAPI (blue); × 20 objective. e The percentage of Nanog- or Oct4-positive cells in blastocysts was quantified based on the staining in d. n, number of embryos analyzed. *p < 0.05, two-way ANOVA