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. 2019 May 29;7:e7001. doi: 10.7717/peerj.7001

Figure 1. Comparison of syringe-infiltration and vacuum-infiltration methods with the TRV empty vector.

Figure 1

(A) Three-year-old P. ostii plantlets at 4 weeks post germination used for agro-infiltration. (B) Schematic depiction of Agrobacterium-mediated TRV inoculation in P. ostii plants using syringe and vacuum methods. (C) The P. ostii leaves subjected to syringe- and vacuum-infiltration with TRV empty vector. (D) Semi-quantitative RT-PCR analysis of TRV1 and TRV2-1 accumulation levels in TRV empty vector-inoculated leaves by syringe and vacuum methods. 18S-26S internal transcribed spacer (18S-26S ITS) was used as internal standard.