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. Author manuscript; available in PMC: 2020 Jan 24.
Published in final edited form as: J Med Chem. 2018 Dec 10;62(2):448–466. doi: 10.1021/acs.jmedchem.8b00909

Figure 6.

Figure 6.

Western blotting analysis of the effect of MDM2 degraders linked from three different tethering sites of lenalidomide on MDM2 and p53 protein levels in RS4;11 cell line. The MDM2 inhibitor MI-1061 and the MDM2 degrader MD-224 were included as controls. RS4;11 cells were treated for 2 h with each individual compound at the indicated concentrations and proteins were probed by specific antibodies. GAPDH was used as the loading control. MDM2 and p53 levels were quantified using an imager and were normalized over the GAPDH loading control.