Figure 3.
Chromatograms obtained for racemic warfarin on columns that contained entrapped HSA, as prepared by slurry-based or on-column entrapment using various concentrations of HSA. Conditions: sample, 40 μM racemic warfarin, 5 μL; column size, 1.0 cm × 2.1 mm i.d.; flow rate, 0.50 mL/min; temperature, 37 °C. The total solution volume was 400 μL in the entrapment methods represented by the top three chromatograms and 350 μL in the procedure represented by the bottom chromatogram. Other conditions are provided in the Materials and methods. The dashed vertical line shows the mean elution time of sodium nitrate, which was used as a non-retained solute.