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. Author manuscript; available in PMC: 2020 Jun 1.
Published before final editing as: Reproduction. 2018 Dec 1:REP-18-0508.R1. doi: 10.1530/REP-18-0508

Fig. 4.

Fig. 4.

(A) Expression pattern of Med20 in wild-type preimplantation embryos. Actb was used as loading control. Oo, metaphase II oocyte; Zy, zygote; 2C, 2-cell embryo; 4/8C, mix of 4- and 8-cell stage embryos; Mo, morula; Blas, Blastocyst. (B) Simultaneous extraction of both RNA and DNA from single blastocyst to perform both genotyping PCR and Med20 RT-PCR, confirming KO was successful. Actb was used as loading control. (C) Endogenous Med20 mRNA was significantly depleted by 3 distinct siRNAs after microinjection. (D) KD of Med20 using distinct siRNAs did not affect blastocyst formation or morphology, but significantly altered embryo outgrowth potential. Red and blue dashed lines indicate ICM colony and trophoblast cells, respectively. Control: scrambled siRNA. n, number of embryos; *, P < 0.05. Scale bars, 100μm.