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Knock-down of Med20 by 3 distinct siRNAs did not affect the expression or localization of either Sox17 (primitive endoderm marker) or Cdx2 (trophectoderm marker), but resulted in ectopic expression of Nanog in Cdx2-positive TE cells. Blastocysts in this experiment were harvested at 4 days post microinjection, and then fixed for immunofluorescence. Scale bar, 50μm.
