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. Author manuscript; available in PMC: 2020 Jun 1.
Published before final editing as: Endocr Relat Cancer. 2018 Dec 1:ERC-18-0478.R1. doi: 10.1530/ERC-18-0478

Figure 3. Silencing of circNF1 inhibits GC cell proliferation and migration but promotes apoptosis.

Figure 3.

A, Schematic representation of backsplice junction and sequence of siRNA targeted to junction site of circNF1. B, Results of qRT-PCR for circNF1 in MKN28 and NCI-N87 cells treated with either si-NC or si-circNF1. C and D, Results of WST-1 assay for proliferation after transfection with si-NC or si-circNF1. Inhibition of proliferation by knockdown of circNF1 in MKN28 and NCI-N87 cells at day 5 (p=0.0079 and p=0.0212, respectively). E, Representative images and quantification results of colony formation of knock-down circNF1. F, Representative images of scratch assays in MKN28 and NCI-N87 cells transfected with control or circNF1 siRNAs. G, Line chart depicting scratch healing rates of MKN28 and NCI-N87 cells after silencing of circNF1. At hour 48t, wound closure in si-circNF1 group is significantly lower than in si-NC group in both MKN28 and NCI-N87 cells (P=0.0196 and 0.0188, respectively). H, Representative images and quantification results of flow cytometry to evaluate apoptosis induction by circNF1 in MKN28 and NCI-N87 cells transfected with si-NC or si-circNF1. *, p<0.05; **, p<0.01; ***, p<0.001.