Fig. 3.

Identification of homozygous ahFAD2B mutant allele a AS-PCR (Yu et al. 2013) for non-mutant ahFAD2B allele (557 bp) identification; b AS-PCR (Yu et al. 2013) for mutant ahFAD2B (441_442insA; 539 bp) allele identification; c CAPS assays for selection of heterozygous and homozygous (441_442insA) alleles; Lanes: M (100 bp DNA ladder); C (Control with water); P1 (GPBD4); P2 (SunOleic95R); 3, 5, 7, 8 (lines homozygous for theahFAD2B mutant allele); 1, 4, 9, 12–15 (lines heterozygous for the ahFAD2B mutant allele); 2, 6, 10,11 (lines homozygous for the ahFAD2B wild allele)