Table 1.
Details of true hybrids, and homozygous ahFAD2B mutant lines obtained in each generation
| Target | Generation | Seeds obtained | Plants germinated and screened | Plants found positive/homozygous | Season |
|---|---|---|---|---|---|
| Hybrid identification | F1 | 8 | 6 (75.0%) | 04 (66.7%) | Summer-2014 |
| BC1F1 | 6 | 5 (83.3%) | 03 (60.0%) | Kharif-2014 | |
| BC2F1 | 6 | 5 (83.3%) | 03 (60.0%) | Summer-2015 | |
| BC3F1 | 10 | 8 (80.0%) | 05 (62.5%) | Kharif-2015 | |
| Total | – | 30 | 24 (80.0%) | 15 (62.5%) | – |
| Selection of ahFAD2B homozygous mutant | F2 | 56 | 52 (92.9%) | 09 (17.3%) | Kharif-2014 |
| F2 (cutting) | 148 | 112 (75.7%) | 18 (16.1%) | Summer-2015 | |
| BC1F2 | 62 | 58 (93.5%) | 08 (13.8%) | Summer-2015 | |
| BC1F2 (cutting) | 70 | 69 (98.6%) | 10 (14.5%) | Kharif-2015 | |
| BC2F2 | 40 | 38 (95.0%) | 04 (10.5%) | Kharif-2015 | |
| BC3F2 | 86 | 83 (96.5%) | 15 (18.1%) | Summer-2016 | |
| Total | – | 462 | 412 (89.2%) | 64 (15.5%) | – |