Fig. 1.

Flow cytometric characterization of populations of CNS immune cells in the tMCAO model. a Experimental design and flow cytometric gating strategy: Following tMCAO, CNS-MPs were acutely isolated at 30 min, 24 h, 48 h, 72 h, and 7 days timepoints and characterized by flow-cytometry based on CD11b, CD45, and Ly6c expression. In independent experiments with viability indicators, viability of > 99% was confirmed using this gating strategy. b Comparison of CD45^high cells within CD11b⁺ CNS-MPs. Quantitative analysis is shown on the right. c Subpopulations of CD11b⁺ CNS-MPs based on CD45 and Ly6c expression and temporal patterns post-tMCAO. An example of CD11b⁺ subpopulations from the ischemic hemisphere at 72 h post-tMCAO is shown on the left. Quantitative analysis of relative proportions of Ly6c^high and Ly6c^low subsets of CD11b⁺CD45^high cells in ipsilateral and contralateral hemispheres at various timepoints is shown. N = 4–8 mice/group, error bars represent standard error of mean. *indicates comparisons between hemispheres (*p < 0.05, **p < 0.01, ***p < 0.005). ^#indicates comparisons within ipsilateral hemispheres across timepoints using 30 min timepoint as reference (^#p < 0.05, ^##p < 0.01, ^###p < 0.005)