Fig. 5.

Alterations in phagocytic capacity of CNS myeloid subpopulations following tMCAO. a Acutely isolated CNS-MPs were incubated with PE-conjugated microspheres and labeled with CD11b-APC-Cy7 and CD45-FITC antibodies. Uptake of > 1 microsphere (phagocytic index) was measured by flow cytometry. Typical phagocytic indices for all CD11b⁺ CNS myeloid cells as well as CD45^low and CD45^high subpopulations at 48 h post-tMCAO (ipsilateral hemisphere) are shown (right). b, c Results from comparison of phagocytic capacity of all CD11b⁺ CNS-MPs as well as CD45^low and CD45^high subpopulations in ipsilateral and contralateral hemispheres following tMCAO. For all analyses, n = 3–4 mice/group, error bars represent standard error of mean, *p < 0.05, **p < 0.01